Generation of biocompatible surfaces
We offer diverse solutions to modify inorganic surfaces such as glass, plastic, metal, graphene, polymers, etc to enable coating with desired proteins for charactereizatio and bioanalysis. We have successfully used several methods including silanization, PEGylation, lipid coating, protein coating and polymer coating. We will work with you to create an ideal biocompatible surface for optimal performance of your preferred analytic platforms.
Sample applications
Integral membrane proteins on Graphene sensor
We worked with Nanotech Biomachines, Inc. to enhance biocompatibility of their graphene based label-free sensors , which allowed the platform to be used for a variety of integral membrane proteins. These proprietary biomimetic membranes allowed determination of kinetic binding data of membrane-bound proteins in their native environment without compromising biosensor performance. Proteins we worked with included monocarboxylate transporter 1 (MCT1), Adenosine A2A Receptor, and Protein A.
Synchronize orientation of proteins for second harmonic generation (SHG)
We worked with Biodesy, Inc. to optimize performance of glass surfaces in their flagship product, the Delta System, such that proteins of interest are uniformly and angularly oriented. This is required for successful measurement of changes in SHG due to conformational change induced by ligand binding. We made a variety surface modifications using lipids, PEG, sugars and proteins to deposit molecules like Ras, Src, Lactadherin, Apolipoprotein E, SemiSWEET, DNA, etc for multiple research applications.